A new technique developed by researchers at Lawrence Livermore National Lab promises to improve accuracy and lower costs of real-time assessment of kidney function, reports an article published this week by SPIE, the international society for optics and photonics, in the Journal of Biomedical Optics.
The paper, published May 3 and accessible via open access, explores the use of multimodal autofluorescence and light scattering to evaluate functional changes in the kidneys after ischemic injury. Conditions including accumulated arterial plaque or blood clots restrict the flow of oxygen and glucose to organs, and prolonged periods of such ischemia can compromise function.
In “Predictive assessment of kidney functional recovery following ischemic injury using optical spectroscopy,” the authors report on their evaluation of various optical signatures to predict kidney viability and suggest a noncontact approach to provide clinically useful information in real time.
While other current work in this area uses expensive multiphoton and laser-based techniques, the authors reduced expenses by switching to camera-based imaging.
Currently, there is no real-time tool to measure the degree of ischemic injury incurred in tissue or to predict the return of its function. The inability to decisively determine tissue functional status runs two great risks: that dysfunctional tissue may be transplanted, increasing the morbidity and mortality of the patient; and that much-needed functional kidney tissue may be discarded.
In their study, Rajesh Raman of Lawrence Livermore National Lab and co-authors Christopher Pivetti and Christoph Troppmann of the University of California Davis, Rajendra Ramsamooj of California Northstate University, and Stavros Demos of Lawrence Livermore acquired autofluorescence images of kidneys in vivo under 355, 325, and 266 nm illumination. Light-scattering images were collected at the excitation wavelengths while using a relatively narrow band light centered at 500 nm.
The images were simultaneously recorded using a multimodal optical imaging system. The recorded signals were then analyzed to obtain time constants, which were correlated to kidney dysfunction as determined by a subsequent survival study and histopathological analysis.
Analysis of the light-scattering and autofluorescence images suggests that variations in tissue microstructure, fluorophore emission, and blood absorption spectral characteristics, combined with vascular response, contribute to the behavior of the recorded signals. These are used to obtain tissue functional information and enable the ability to predict post-transplant kidney function.
This information can also be applied to the prediction of kidney failure when visual observation cannot, almost immediately following an injury.
Reviewers of the study suggested other promising applications for future development, and envisioned this approach being used as a screening tool for assessing kidney viability prior to transplant. In particular, they say, these cost-effective screening methods could benefit healthcare in developing countries.
Multimodal imaging also has provided insights into other physiological events that may occur during ischemia and reperfusion.
“This work’s exceptional value lies in the realization of a workable practical system that has excellent potential to be adopted in field situations,” says journal associate editor Andreas Mandelis (University of Toronto).