A tiny motor tasked with one of natures biggest jobs is now better understood. The molecular machinery that helps export messenger RNA from a cells nucleus has been structurally mapped at the Advanced Light Source, a synchrotron located at the U.S. Department of Energys Lawrence Berkeley National Laboratory (Berkeley Lab).
Messenger RNA conveys genetic information from the nucleus to the cells cytoplasm, where it guides the synthesis of proteins – the workhorses of biology. A key protein complex that helps to ferry messenger RNA from the nucleus has been poorly understood until now, however.
“Our research describes how this protein complex works at the molecular level,” says Ben Montpetit, a postdoctoral researcher in Karsten Weis lab at the University of California, Berkeley. Their research, a collaboration with biochemists Nathan Thomsen and James Berger, also of the University of California, Berkeley, is described in a paper published March 27 in an advance online edition of the journal Nature. Berger is also a faculty scientist in Berkeley Labs Physical Biosciences Division.
The scientists studied a protein called Dbp5 that resides at the nuclear pore complex of fungi, plant, and animal cells. In these organisms, it reshapes messenger RNA as part of a chain of events required to send it from the nucleus.
But thats just the tip of the iceberg. Dbp5 is among a class of enzymes called DEAD-box ATPase that perform vital RNA-remodeling functions throughout nature, from humans and oak trees to fungi and single-celled bacteria. Understanding how it works in the cells of one species will illuminate how it works in distantly related species.
“DEAD-box proteins are conserved throughout life, so learning how it works in this case sheds light on its function everywhere in nature,” says Montpetit.
The scientists conducted their research at beamline 8.3.1 of the Advanced Light Source, a national user facility that generates intense x-rays to probe the fundamental properties of substances. They used the synchrotron to resolve the structure of Dbp5 from yeast cells at key steps of the enzymes job, such as when its activated by another protein called Gle1 and when it binds with RNA. The structures were obtained at resolutions of between one and four angstroms (one angstrom is the diameter of a hydrogen atom).
The result is a time-lapse series of the proteins choreographed bid to remodel messenger RNA, with its twists and turns revealed at the highest resolution yet.
Among the teams most intriguing discoveries is the role of a molecule that is known to be involved in messenger RNA transport, but whose function was a mystery. They found that the molecule, called inositol hexakisphosphate, or IP6, tethers Gle1 to Dbp5. This stabilizes the two proteins long enough for Gle1 to kickstart Dbp5 into action.
“IP6 acts like a molecular glue,” says Montpetit. “This is one of the first examples of an endogenous small molecule functioning to bring larger protein molecules together. With this knowledge, scientists can now consider how IP6 may be used to regulate mRNA export under various conditions, such as in response to stress.”
Their research could also advance scientists understanding of a rare but devastating family of diseases called lethal congenital contracture syndrome. The mutation that causes this disease is mapped to the genes that produce both Gle1 and IP6. Now, with Gle1s role in messenger RNA transport further elucidated, the door opens for the development of therapies that target its function.
The research was funded by the National Institutes of Healths National Institute of General Medical Sciences and the G. Harold and Leila Y. Mathers Foundation.
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